Thu, 09 Jul|
Webinar with Dr Shayne Mason, on NMR metabolite profiling
Time & Location
09 Jul 2020, 12:00 SAST
About the Event
The major challenge in metabolomics is to identify the metabolites in a highly complex mixture of compounds that produce a forest of peaks in a NMR spectrum. This metabolite identification process, called metabolite profiling, involves fitting the mixture spectrum to a set of individual pure reference spectra obtained from known pure compounds. If done correctly, the fitting process yields not only the identity of the metabolites, but also the accurate concentration of those metabolites. Therefore, the end result of a successful metabolite profiling study is a table of metabolite names and their absolute or relative concentrations. This webinar will provide a practical stepwise guide describing how to perform 1H-NMR metabolite profiling on multiple complex biological samples. This protocol is then applied to an example of a typical untargeted 1H-NMR metabolomics study.